Composite

Part:BBa_K2387025

Designed by: Stijn Prinsen   Group: iGEM17_Wageningen_UR   (2017-11-01)


Inducible tethered CpxP-Affinity molecule

This composite biobrick contains a fusion of MBP misfolder MalE24-1, Cpx system inhibitor CpxP, and an affinity body specific for IgG Fc region. This fusion is controlled by inducible promoter ptac and contains a strong RBS. In the Mantis project of Wageningen iGEM 2017 it was used to create a membrane sensor for presence of antigens in the blood of a patient. This fusion can theoretically bind the IgG Fc region, leading to alleviation of the Cpx suppression by CpxP as it dissociates from the membrane sensor CpxA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 522
    Illegal BglII site found at 1440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 220


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Categories
Parameters
None